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KMID : 1234420110390030301
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Korean Journal of Microbiololgy and Biotechnology
2011 Volume.39 No. 3 p.301 ~ p.307
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Bioremediation Efficiency in Oil-Contaminated Soil Using Microbial Agents
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Hong Sun-Hwa
Lee Sang-Min
Lee Eun-Young
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Abstract
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Oil pollution is a world-wide and prevalent treat to the environment. Physic-chemical remediation technology, used with total petroleum hydrocarbon (TPH) contaminated soil, has proven to be very slow and uneconomic. Bioremediation of contaminated soil has been seen to be a more efficient method where the concentrations of oil are moderate and non-biological techniques are not economical. The aim of this research was to investigate the influence of additives on TPH degradation in a diesel contaminated soil environment. Six experimental conditions were conduced; (i) diesel contaminated soil, (ii) diesel contaminated soil treated with microbial additives, (iii) diesel contaminated soil treated with microbial additives where the mixture was titrated to an end point of pH 7 with NaOH, (iv) diesel contaminated soil treated with microbial additives and accelerating agents, and (v) diesel contaminated soil treated with microbial additives and accelerating agents where the mixture was titrated to an end point of pH 7 with NaOH. After 10 days, significant TPH degradation (67%) was observed in the DSP-1 (v) soil sample. The removal of TPH, in the soil sample where microbial additives were supplemented, was 38% higher than the control soil sample during the first ten days. The microbial additives were effective for both the initial removal rate and the relative removal efficiency of TPH when compared with the control group. However, various environmental factors, such as pH and temperature, also affected the activities of microbes living in the additives, so pH calibration of oil-contaminated soil would help the initial reduction efficiency in the early stages.
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KEYWORD
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Bioremediation, denaturing gradient gel electophoresis (DGGE), microbial agents, oil-contaminated soil
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